Investigation Theories Of The Problems Of Healthcare Quality Coursework

Investigation Theories Of The Problems Of Healthcare Quality - Coursework Example These strategies will make inquiries from study designs like open-ended question interviews (Handout, 2011). This is specifically inclined towards either pre or post aspects of behaviors and traits. The population to be is quite fundamental and important because, the purpose supports qualitative theories and phenomenology theory, which is of use in supporting phenomenal experiences (Creswell & Stone, 2009). The population also acts to determine scenarios when the investigators can support the general process. Will the population from the qualitative research test the method from both the phenomenology and ground theories the directions taken by health disparities because of the causes by the large chunk of the population without the health insurance? How would this issue be addressed taking note that the large portion cannot access medication and healthcare hence resorting to crude measures to support health? From the phenomenological perspective, a design is created on a mirrored concept from the university current online education framework. The ground theory as stated will also be considered due to the need for quality and reliability of data. This is the ground upon which the researcher is able to reinforce the open process, action or integration because of the education concept. According to the Quality Health Care QHC use, it is if training and development through education to help improve the talent from the family care perspectives to understand the need for quality healthcare and subscribe to health insurance policies (Shadish, Cook & Campbell, 2002). The researcher’s strategy is to support personal development theory, which is directed towards promoting community and change in leadership.  The vision to judge and design ways to adopt modern development strategies to perpetuate values aimed at planning the workforce, community, and culture change in order to promote the skills by individuals to mitigate the healthcare problem.

Organizational Knowledge Essay Example | Topics and Well Written Essays - 1750 words

Organizational Knowledge - Essay Example Other than this, in this competitive scenario, the essence of retailing changed adversely and gave rise to a centralized market situation where the rate of exchanged is extremely high as compared to others (Feldman, 2003). In this market, metal coins and paper notes are also exchanged for other goods which enhanced the scope and extent of retailing to a high extent in the recent decades. Side by side, introduction of online stores also enhanced the range of total sales in retaining sector in today market scenario (Moser &Vander, 2003). However, in spite of huge advancement, the Retail Management sector presented numerous interesting challenges that gave rise to more innovative solutions. The most vital and noteworthy issue that is hampering the portfolio of the sector of retaining is commoditization. It is a trouble-some issue that arose a few decades before that mainly highlights price wars and fierce competition among the rival players of the market. Due to which the position and b rand image of numerous reputed organization operating successfully in this segment is experiencing massive downfall resulting in reduction of the profit margin and total sales of GameStop Corporation of US. As a result, the position of GameStop Corporation in the markets of US is reducing at a significant extent, which is quite insignificant (Moser &Vander, 2003). ... This cause also marked a significant downfall of the position of US among other big players around the globe (Moser &Vander 2003). Detailed background information of the issue of commoditization described above Historical Origin of the problem: Due to the entrance of numerous new entrants, the extent of competition aroused, resulting in the problem of commoditization in the segment of retailing. Other than this, as entry in this segment do not require huge rate of investment and so it’s quite easy for the new entrants to enter it (Moser &Vander, 2003). As a result, the segment became extremely aggressive and the rate of switchovers also increased to a significant extent (Nonaka & Nishiguchi, 2001). In case of pricing problem or lack of trendiness, the customers immediately shift over towards other brands resulting in loss of prospective customers and profit margin as well (Moser &Vander, 2003). In order to cope up with this problem, I instructed my juniors and other administra tive officials to maintain a competitive margin of the products so as to retain the customers towards their brands and products. Along with this, I also decided to introduce ad present more concentration in introducing varied types of innovative designs and features in the products lines so as to make the customers attracted towards their products as compare to other rival players (Moser &Vander, 2003). Otherwise, they might not be able to maintain a sustainable position in the market among other players (Tsoukas, 2005). This proved quite successful for the organization thereby amplifying the profit margin (Moser &Vander, 2003). Proposed Solution Not only this, according to Cooper, (1999), to maintain the portfolio of the products, just like the other retailers I also tried to implement the

Criminology -Differential Association + Institutional Anomie Theories Essay

Criminology -Differential Association + Institutional Anomie Theories - Essay Example Agnew assumes that individuals from all social classes engage in crime, as all are geared towards success and economic fulfilment. In fact, the general strain theory should not be confined to economic fulfilment as whether from a low social class or higher, individuals would rebel if what they own is taken away from them and their success therefore hampered or in cases of negative emotions. In such a case therefore, the poor will have to steal or rob in order to recover the lost property or be at the same social level with the rest of the members of the community, while those in a higher social class would engage in fraud or embezzlement of funds, in most cases at the work place so that they can keep being rich; so as to be able to deal with the negative emotions that are as a result of the strain they experience. He further argues that an individual’s inability to achieve the success they aspire, in terms of money can lead to strain and therefore push someone into engaging in crime. He also says, â€Å"Many middle and upper class people in the United States want more money than they can have and obtain through legitimate channels†. Therefore, this strain on an individual to attain monetary success is what pushes them to engage in white-collar crime so that they can deal with the strain completely; mostly in cases where they are not offered solutions for the strain they are experiencing. An individual may for instance fake various documents in order to land a new lucrative job they have always wished to have. This is fraud, which is a white collar-crime. In relation to the general strain theory, white collar crime, just like any other criminal activity is as a result of the negative emotions one has when what they want or need is not being achieved. Fraud and embezzlement of funds for instance in most cases takes place in

Crew Resource Management Research Paper Example | Topics and Well Written Essays - 1250 words

Crew Resource Management - Research Paper Example This research paper describes ways of implementing successful crew managment tasks for organizations. Crew resource management is the study of the behavior of crew members in industries of aviation, fire related services, marine industries and other such types of industries. The origin of crew resource management took place in the year 1979 in a workshop that was conducted by NASA. The purpose of crew resource management is to train, develope and increase the effectiveness of the crews, such as firefighers, police crews, troops and other military forces. Research paper defines that the main accomplishments that the crew resource management focuses on are the adaptability skills, the assertiveness skills, the mission analysis experience, communication job, leadership art, decision making competence and also developing the skills of situational awareness. The leadership arts that are required in the phase of crew resource management are to focus on coordinating and directing the activities of the entire crew team. The crew members need to be motivated by their leader at all times and should be insisted towards showing positive team work at all times. This research paper also covers basic requirements that are needed for a leader to provide all types of assistance and information to the team members so that all, for example, military operations can take place smoothly.

Voices of freedom Journal Industrialization Essay Example for Free

Voices of freedom Journal Industrialization Essay Industrialization, †the new economy† brought on a wave of new movements and activism in America. Labor movements, women’s rights activists, social, religious, and political reform are among the changes of the Market Society. Industrialization shaped Market Society expansion in many ways. Immigration, Nativism, Westward Expansion, the rise of Nativism,Transcendentalists Movement and The Second Great Awakening all taking place during the 1800-1840 Market Society growth. Market Society refers to farmers, large city growth, factory system, and immigration. Industrialization was the force that shaped Market Society. In the Northwest farming and commercial cities were booming. Westward expansion was due to land being cheap and plentiful which was especially appealing. The belief that freedom laid in the west was because of the availability of land and was ultimately important to economic independence. Here farmers sold livestock and were able to rapidly plow land with aid of the steel plow. While credit and the market was to the east. Cities like Cincinnati and Chicago would grow due to the population growth from immigration and high demand for jobs. Artisan workers were soon replaced by that of the factory worker usually female or immigrants. The very first factory was established in 1790 based on an outwork system. Later large scale factories would be constructed focusing on cotton textiles. Lowell factory is known for their female factory workers. These women adopted the name â€Å"Lowell† girls. Lowell girls were young women of farm families who were offered meager wages for long hours of labor. Ultimately overworked and frustrated workers would expose the grueling conditions of working in the factory. In the words on one Lowell factory worker document 51 in Voices of Freedom, Eric Foner â€Å"Shall they be compelled to listen in silence to those who speak for gain, and are the mere echo of the will of  the corporations? Shall the worthy laborer be silenced by wealth and power, and for fear of being deprived of the means of procuring his daily bread?†. Written is 1845 this worker was upset and speaking out after being over worked, mistreated, and underpaid. This quote is telling not only of her personal feeling about being silenced by power but the mass feelings of injustice to the common worker by corporations at the time. I feel the audience this worker is trying to reach is not only fellow workers to gain backing in resistance of mistreatment but the corporations as well. She is standing up for her own integrity and encouraging others in her situation to the same. She is rightfully casting shame on those corporations for inflicting those conditions. It is during this time that women began to fight for equal rights of opportunity and happiness outside of the home. Immigration caused labor demands to rise fueling the economic expansion. The approximately 4 million immigrants who migrated to America had a variety of motivating factors. Irish Potato Famine, European economic conditions, religion and politics are all some of the reasons immigrants were making the voyage to the America in such large numbers. Most immigrants during this time were German or Irish. Generally Germans came to America more skilled workers and the Irish were unprepared fleeing famine forcing them to fill low-wage laboring jobs. Eventually Nativism would rise in the 1840’s and 50’s. A racially fueled violent backlash in New York City and Philadelphia against immigrants, â€Å"Nativists† feared immigrants were the down fall of America. Nativists blamed immigrants for crimes, corruptions, addictions, and poor wages. ‘Walks among the New york City Poor’, New York Times June 23,1853. Document 52. Voices of Freedom, Eric Foner. â€Å"Healthy, stout frames, and low, degraded faces with many stamps of inferiority†. Here the writer really captures the discriminatory American opinion of immigrants at this time. Nativism labeled these people as inferior and not capable of rising above the status that they arrive with. He is stating that immigrants are seen as inferior to everyone around them and in for a hard time as the reading goes on. I feel like the purpose of the article is to shed light on the high hopes of immigrants and minimal possibility in the new world. This was a very difficult time to be an immigrant. During this time a group of New England intellectuals formed called The Transcendentalists. This group of men believed in the idea of The Free Individual. Ralph Waldo Emerson was a Transcendentalist who believed freedom obtained by the process of self realization transforming ones self according to their own liking. Voices of freedom, Eric Foner document 54. â€Å"The American Scholar 1837,† Ralph Waldo Emerson opens his powerful statement â€Å"In self-trust, all the virtues are comprehended†. Ralph Waldo Emerson addresses the nation and President directly. He is expressing here that self realization is the key success and freedom. This was the fundamental principle of Individualism the the Transcendentalist’s believed in. Henry David Thoreau also a Transcendentalist believed the market revolution worsened individual judgment and freedom was within the individual. The Second Great Awakening was more optimistic than the first and had similar philosophy of the Tra nscendentalists with the addition of a religious twist. The belief in self improvement through determination with the addition of Christianity. Christian denominations such as baptists and methodists began to branch off during this time.

14-3-3 Protein Analysis | Essay

14-3-3 Protein Analysis | Essay INTRODUCTION 14-3-3 represents a family of highly conserved, 28-33 kDa, acidic dimeric proteins. [B, H1] These proteins were first identified and isolated by Moore and Perez during their systematic study of protein fractions from mammalian brain tissue. [B1] Their nomenclature reflects the system used by the researchers to classify the various protein fractions, based on their migration pattern on starch gel electrophoresis and two-dimensional DEAE chromatography. [D1] Initially it was thought that the occurrence of these proteins was confined to neuronal tissue, where they were found to be present in concentrations as high as 13.3 ÃŽ ¼g/ml. [B19] However, subsequent studies showed that they are in fact widely distributed and ubiquitously expressed in most mammalian tissues. [B] Apart from mammalian tissue, orthologous proteins with a high degree of similarity have also been identified in other organisms including plants, yeast, insects and amphibians. [B] Interestingly, though no prokaryotic ancestor has been identified for the 14-3-3 gene, eukaryotes are known to express multiple isotypes, encoded by highly similar gene sequences. [A, G13] Drosophila and yeast each contain two 14-3-3 genes, Arabidopsis species have 15, while the human genome contains seven closely related 14-3-3 genes. [G13-G16] These genes encode the seven different 14-3-3 isotypes found in human cells – namely ÃŽ ², ÃŽ ³, ÏÆ', ÃŽ µ, ÃŽ ·, Ï„, and ÃŽ ¶. Two additional isoforms – ÃŽ ± and ÃŽ ´, represent the phosphorylated varieties of ÃŽ ² and ÃŽ ¶ respectively. [D7] By virtue of their conserved sequences, the different 14-3-3 isoforms display a similar tertiary structure. Crystallographic studies show that each subunit is made up of nine anti-parallel ÃŽ ± helices, which are able to self-assemble into dimers [F1]; with four of the nine helices participating in this process of dimerization. [A] While certain isoforms such as ÏÆ' and ÃŽ ³ show propensity towards the formation of homodimers, other isoforms like ÃŽ µ prefer to heterodimerize. [H] Structural analyses have also revealed that each monomer contains an amphipathic concave channel through which it interacts with its target proteins. [F13] Since each of the subunits of the dimer contain an independent ligand-binding groove, these proteins can interact with two different binding sites present on the same or different target proteins [H3] The targets of 14-3-3 proteins were first studied by Muslin et. al. in 1995, who determined that these proteins interact with phosphorylated serine-containing binding partners in a sequence specific manner. [A2] Thus, this came about to be the first family of proteins discovered to have an affinity for phosphoserine-specific targets. More specifically, Yaffe et. al. in 1997 identified two putative high-affinity motifs, bearing the sequences RSXpSXP and RXXXpSXP, where pS stands for phosphoserine, R stands for Arginine, P is proline and X is any amino acid. [] These were found to be the consensus sequences for 14-3-3 binding, and were called the mode I and mode II binding motifs respectively. [A21] However not all the 14-3-3 binding partners conform to these motifs and neither are all 14-3-3-target interactions found to be phosphorylation-dependent. [H] Though, a small percentage of 14-3-3 targets do not conform, in general it can be said that, proteins that interact with the 14-3-3 family are usually found to be globular proteins that contain either the mode I or mode II binding motifs within an unstructured region of the peptide. [PG] Interestingly, despite the fact that the different isotypes share a similar structure and show complete conservation of sequence in the ligand-binding region, not all the isotypes of 14-3-3 bind equivalently to their ligands in vivo and the pathways in which the individual isoforms participate have diverged considerably. This isoform specificity cannot be explained solely on the basis of 14-3-3 binding to the consensus sequence. [A, PA] It has hence been speculated that this isoform-specificity may be attributed either to differences in subcellular localization and/or tissue-specific transcriptional regulation rather than inherent differences in their ligand-binding ability. [D] Consequently, the various isoforms of the protein are found to bind different ligands and hence influence distinct cellular pathways and processes. Some of the ligands postulated on the basis of database searches include Raf1, CDC25C, polyoma middle T antigen, BAD, 5’AMP Kinase and PLC ÃŽ ³. [PA] Studies conducted in the late 1990’s demonstrated the ability of 14-3-3 proteins to interact with a number of different protein phosphatases and kinases in the cell, thus influencing multiple signalling pathways within the cell. [D] The mechanism of action of the 14-3-3 family of proteins is based on their ability to alter the stability, the catalytic activity or the subcellular localization of their targets. [H] 14-3-3 dimers being highly rigid, they are able to induce conformational changes in their target proteins as well as generate steric hindrance upon binding. Together, these forces can prevent molecular interactions by modulating the accessibility of the ligand to enzymes; and they can also serve to expose or hide localization motifs such as NES and NLS. [H] The first cellular activity to be attributed to 14-3-3 was that of an activator of tryptophan and tyrosine hydroxylases, rate-limiting enzymes that regulate the biosynthesis of catecholamine and serotonin neurotransmitter, as reported by Ichimura and co-workers. [B20] Subsequently it was discovered that 14-3-3 proteins could regulate the activity of signal transduction molecule protein kinase C. [D3,D4] This and other findings led to the implication that 14-3-3 proteins could act as novel chaperone proteins that are able to modulate the interactions between the different constituents of signal transduction pathways. [D5] Today it is known that 14-3-3 protein dimers are able to interact with a wide array of proteins within the cell including signalling molecules, apoptosis factors, tumor supressors, transcription factors, biosynthetic enzymes as well as cytoskeletal proteins; as a result of which they play crucial roles in the regulation of multiple cellular process such as the onset of cellular differentiation, and senescence, DNA repair and the maintenance of cell cycle check point, co-ordination of motility and adhesion and the prevention of apoptosis [A,H] REVIEW OF LITERATURE 14-3-3 proteins are crucial in the regulation of many cellular functions. An implication of their involvement in this multitude of processes is that any mutation, loss of regulation or altered expression of these proteins can lead them to be associated with major diseases. Through their involvement in the regulation of various tumor suppressor genes and oncogenes, 14-3-3 proteins are thought to be potentially involved in cancerous transformation and the development of malignancies. [Z11] They are also known to be associated with neurodegenerative conditions such as Alzheimer’s disease, ataxia and Parkinson’s disease. [MT1, MT2, MT3, MT4] The causes of these diseases are poorly understood and hence this family of proteins has been the focus of study of several research groups around the world, leading to numerous publications investigating all the different aspects. This section summarizes the literature elucidating the properties and functions of 14-3-3 proteins that have helped shape the rationale behind this project. A special focus is maintained on current literature that highlights role of 14-3-3 ÃŽ ³ in the cell cycle and in desmosome assembly, as well as its recently discovered ATPase activity. Human 14-3-3 isoforms The members of the 14-3-3 family are among the most abundant proteins in the cell. It has been established that owing to their interaction with diverse targets, 14-3-3 proteins are known to be involved in a multitude of processes, including the control of gene transcription, metabolism, cell cycle regulation, and apoptosis. [C] However, not all 14-3-3 proteins carry out the same functions. The table below provides an overview of the properties of the various human 14-3-3 isoforms: 14-3-3 isoform Official symbol Official full name Chrom-osome location No. of amino acids Mol.Wt. (kDa) Interactions, functions and disorders 14-3-3 ÃŽ ² YWHAB Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta 20 q13.1 246 27.95 shown to interact with CDC25 phosphatases and RAF1 may play a role in relaying mitogenic signaling to the cell cycle machinery 14-3-3 ÃŽ ³ YWHAG Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma 7 q11.23 247 28.12 interacts with RAF1, CDC25C and protein kinase C induced by growth factors in human vascular smooth muscle cells also expressed significantly in heart and skeletal muscles 14-3-3 ÏÆ' YWHAS, SFN Stratifin 1 p36.11 248 27.64 known to interact with PLK4, ERRFI1, MARK3, JUB expressed in epithelial cells 14-3-3 ÃŽ µ YWHAE Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon 17 p13.3 255 29.03 interacts with RAF1, CDC25 phosphatases, and IRS1 proteins plays a role in pathways related to signal transduction, cell division and regulation of insulin sensitivity implicated in the pathogenesis of small-cell lung cancer 14-3-3 ÃŽ · YWHAH Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta 22 q12.3 246 28.09 contains a repeating sequence, 7 bp in length, in its 5 UTR changes in the number of repeats are associated with conditions such as psychotic bipolar disorder and schizophrenia 14-3-3 Ï„/ÃŽ ¸ YWHAQ Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta 2 p25.1 245 27.63 found to be over-expressed in patients with amyotrophic lateral sclerosis also expressed in T cells 14-3-3 ÃŽ ¶ YWHAZ Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta 8 q23.1 245 27.61 interacts with IRS1 protein may play a role in regulating insulin sensitivity 14-3-3 ÃŽ ³ 14-3-3 ÃŽ ³ is an adapter protein that is known to be involved in the regulation of a wide range of signalling pathways, both general and specialized. This protein is encoded by a gene designated YWHAG, which was mapped to the chromosome 7q11.23 by Horie et. al. in 1999 [Z2] A study of its binding partners in a yeast two-hybrid study revealed that 14-3-3 ÃŽ ³ may have as many as 130 potential ligands. [PG 4] There are reports which link the overexpression of 14-3-3 ÃŽ ³ and its high titres in the cerebrospinal fluid (CSF) with certain neurodegenerative disorders such as Down syndrome and Alzheimer’s. [A129, A131] This protein is also thought to to be involved in the process of carcinogenesis. Although 14-3-3 ÏÆ' is the isoform that has been most extensively studied for its association with human cancers, there are also several ongoing studies which attempt to establish a correlation between 14-3-3 ÃŽ ³ expression and the development of tumours. This includes the work of Jieqiang Lv. et. al. which was able to demonstrate through proteomic analysis that a reduced expression of 14-3-3 ÃŽ ³ is observed in patients with uterine tumours. [Z10] The role of 14-3-3 ÃŽ ³ in cancer can be better appreciated by understanding its role in the cell cycle and its regulation. Role of 14-3-3 ÃŽ ³ in the cell cycle 14-3-3 proteins play a major role in the regulation of cell cycle through the interaction with various cell cycle proteins. The main targets for 14-3-3 regulation are the Cdc25 proteins. Cdc25 is a family of protein phosphatases comprising the members Cdc25A, Cdc25B and Cdc25C, which are active during different phases of the cell cycle. Cdc25A participates in the regulation of G1/S transition, whereas Cdc25B and Cdc25C regulate G2/M transition. [z3] Cdc25c has been specifically implicated in the activation of the CDK1-cyclinB1 complex by removing inhibitory phosphorylations at T14 and Y15, which advances the cell cycle from G2 to M phase. The overexpression of this protein has been seen to result in mitotic catastrophe in the cell due to premature mitosis. [E28] Therefore the expression and function of Cdc25C needs to be tightly regulated; and 14-3-3 ÃŽ ³ plays a crucial role in this regulation. [E29] During the interphase, 14-3-3 proteins bind to Cdc25C and sequester it in the cytoplasm by occluding the NLS, preventing it from accessing the CDK1-cyclinB1 complex in the nucleus and thus in essence inactivating it. [E28] Dalal et. al. showed that though all the 14-3-3 proteins were able to carry out this function in vitro, only 14-3-3 ÃŽ ³ and ÃŽ µ were found to bind to and thus inhibit Cdc25C in vivo. [] This binding however was found to require the phosphorylation of a specific serine residue at position 216 in human cells, usually carried out by TAK1 and other similar kinases. [E30, z4] Cdc25C is also known to be a target of the DNA replication and DNA damage checkpoints that exert their effects through the phosphorylation of S216 by kinases like Chk1 and Chk2. [z5, z6, z7] This phosphorylation thus generates a high-affinity motif that is able to bind to 14-3-3 proteins present in the cytosol. A schematic representation of this process is provided in the figure below. In the absence of 14-3-3 proteins, this regulation of Cdc25C is disrupted and a result, cells lose their ability to stop cycling upon loss of integrity of their DNA. To this effect, a study conducted by Hosing et. al. in 2008 showed that cells require 14-3-3 ÃŽ ³ in order to maintain their ability to arrest cells in the S phase and the G2 phase checkpoint. [] This was proven by generating 14-3-3 ÃŽ ³ knockdown cells, and studying their response to DNA damage. It was found that these cells were unable to arrest in G2, thus leading to an increase in premature chromatin condensation (PCC) as compared to the vector control. [] This was the first report suggesting that 14-3-3 ÃŽ ³ modulates the cell cycle checkpoint response, through its negative regulation of cdc25C function. Role of 14-3-3 ÃŽ ³ in desmosome assembly 14-3-3 isoforms were also not known to have any role to play in the assembly of desmosomes, until a serendipitous observation led to a study conducted by Sehgal et. al. This study demonstrated for the first time that 14-3-3 ÃŽ ³ is required for desmosome formation. [] They showed that 14-3-3 ÃŽ ³ is able to form complexes with the desmosomal proteins plakoglobin, desmoplakin and plakophilin 3. Their work led to the conclusion that 14-3-3 ÃŽ ³ is essential for the recruitment of plakoglobin to the cell border; and that the loss of 14-3-3 ÃŽ ³ leads to a decrease in desmosome formation. This translates to defective cell-cell adhesion in HCT 116 cells and sterility in male mice when this loss is manifested in the testis. [] These results are in agreement with the fact that 14-3-3 ÃŽ ³ plays a role in the transport of proteins from the Golgi complex to the cell border, as previously reported by Valente et al. in 2012. [Z1] ATPase activity of 14-3-3 proteins The notion that 14-3-3 proteins may possess an ATPase function evolved through a series of studies. The first of these was reported in a paper in 1993 by Hachiya et. al., who had isolated a protein from the rat liver cell cytosol, called the mitochondrial import stimulation factor (MSF). [] This factor was found to modulate the conformation of aggregated proteins and stimulate their import into the mitochondria in an ATP-dependent manner, much like the Hsp70 chaperone molecule. Ensuing studies by the same group led to the cloning of cDNA sequences encoding the large and small rat MSF subunits; and based on these cDNA sequences, it was deduced that the MSF peptides belong to the 14-3-3 family. [] This was thus the first time the ability to hydrolyse ATP was attributed to a 14-3-3 protein. In 1997, Yano et. al. probed further and asked whether the 14-3-3 family of proteins were also capable of the reverse reaction i.e. ATP synthesis; and found that native 14-3-3 proteins isolated from human lymphoblastoma cells as well as recombinant 14-3-3 Ï„ exhibited the ability to generate ATP from ADP. [] This activity resembled that of the enzyme nucleoside diphosphate-kinase. It was then speculated that ATP synthesis and hydrolysis may play a role in regulating the interaction of 14-3-3 proteins with their substrates. Subsequently, in 2006, Yano et. al. also showed that 14-3-3 ÃŽ ¶ acts as a molecular chaperone under heat shock conditions, dissolving thermally-aggregated proteins, also in an ATP-dependent manner. [] This stress-related function is said to differ from the role of MSF as previously described, since in that case, the protein activity is limited to the transport of newly synthesized proteins into the mitochondria. This finding thus represented another part of the puzzle that is the multi-functional nature of 14-3-3 proteins. Despite these reports however, the ATPase activity of 14-3-3 proteins had not commanded much attention from researchers, until recently Ramteke et. al. in 2014 demonstrated unequivocally, using 14-3-3 ÃŽ ¶ as a model, that these proteins possess an intrinsic ATP-hydrolysing function. [] They also studied the putative residues essential to this activity and found that in their model, the mutation of the Asp at residue 124 to Ala (D124A) resulted in a significant increase in the rate of ATP hydrolysis, while the mutation of Arg 55 to Ala caused a partial loss of function. ATPase activity of 14-3-3 ÃŽ ³ As a part of the same study, Ramteke et. al. decided to test whether the other 14-3-3 isoforms also displayed similar properties; and they found that with the exception of 14-3-3 ÏÆ', all the other isoforms, including 14-3-3 ÃŽ ³, showed intrinsic ATPase activity. [] Interestingly, it was found that in case of the ÃŽ ³ isoform, the mutation of the conserved Asp, found at position 129 to Ala (D129A) led to an over two-fold gain in ATPase function, reflecting that the binding of this mutant to ATP is probably either more energetically or sterically favourable. [] The authors of this paper were however unable to comment on the functional relevance of the ATPase activity of 14-3-3 proteins. These results however provide an impetus to further studies on physiological significance of ATP hydrolysis, with respect to individual 14-3-3 isoforms.

Increasing the Minimum Wage in America

Increasing the Minimum Wage in America Raising the lowest wages in America has always been a controversial topic. The Federal Government establishes the minimum hourly wages that a worker can be paid and as such, these standards of income impact the entire nation.   These standards of income or minimum wages were written into law to control the unfair practices of sweatshops and other capitalistic, manufacturing firms. However, minimum wage laws of today ensure that the employee being paid is provided with enough money to afford necessities (Edward and Gilman, 1999).   Nevertheless, with rising energy costs, no real affordable heath-care system in place and rising fuel and food costs the minimum minimally covers the average Americans necessities. The controversy and or problems associated with standard wages stem from both the positive and negative impacts that it imparts upon society. For example, as minimum wages increase companies are less likely to hire new employees, to train or retool current employees or to off er incentives and or job advancements.   In reverse, as wages decrease firms began to experience higher levels of employee turnover rates, incidents of accidents and often are left with less qualified applicants (Doyle, 2017). Seeing that much of this countries workforce is made up of hourly employees these minimum wage imbalances typically disrupt the main source of income for many households in America.   Ã‚   Technology and various factors of life have changed over the years and has greatly advanced. However, what has remained stagnant; is the increases to minimum wages. As such, many people in America are unable to properly provide for their families. Poverty rates have increased as well as the number of the homeless, yet the Federal Government reports that our economy is improving. Nevertheless, many of this great, Nation’s citizens are unable to provide good and nutritional food for themselves and their families. Because of this we are now facing a rising epidemic of poor health and illness in this country. Due in part to the; depression, obesity, poor nutritional lifestyles brought on by poverty (T.H.G,2014). Throughout the years there has been research that has proven that workers who make more than minimum wage tend to be well and in good health. However, the Federal Government feels that by increased minimum wages create heightened levels of unemployment and that if minimum wages were increased above ten dollars per hour that these rates would elevate drastically. Truth be told, if minimum wages could increase as much as $25 an hour and firms and organizations would continue to grow and prosper because their inputs would always be higher than their outputs. Thriving companies like Amazon wouldn’t stop hiring and neither would they have to raise the prices on their goods to compensate for increased wages. Ideally, if wages were raised it would essentially grow the middle-class and low-income worker would be less reliance upon government assistance programs. As a result, hard working Americas are speaking out and protesting for a substantial increase to the minimum wages. This in essence is warranted because over the last few year’s inflation has increased however income has not. According to different reports given by the Labor Board, â€Å"2007 amendments increased the minimum wage to $5.85 per hour which took effect on July 24, 2007; $6.55 per hour effective July 24, 2008; and $7.25 per hour effective July 24, 2009.† Throughout much of this time America was fighting 3 wars, losing several companies to foreign barters, dealing with a real estate crisis as well as an extreme recession. So, these relatively menial increases were not in alignment with gas prices, inflation nor overall citizen expenses. Statics show at the beginning 2017, twenty-nine states have a higher minimum wage than the standard federal rate. Implementing a minimum wage increase would promote equality among the nation, while also paying workers a living wage which will aid in one being able to live a decent and healthy life. Despite the hesitation from employers, many American households live off minimum wage, for this reason lawmakers should increase it; which will aid American families with the ability to afford the cost of living. Problem The bare minimum of anything is less than average and as such is subpar! Minimum wage in supposedly ‘Greatest and Strongest Nation’ in the world falls closer to the wages extended to citizens in less developed countries. America is ranked number 9 and has a greater military and infrastructure than at least two of the countries that out pay their citizens. Australia which is ranked number one as it relates to highest minimum wages paid to it’s workers and citizens yet this country founded in 1901 and America was founded in 1776, a whooping 275 years earlier (Papworth, 2012). America’s Minimum wage institution was established in 1933, and upon its inception citizens were paid twenty-five cents an hour. Eventually the wages were raised regularly in the years after, with the time between increases generally ranging between a year, and up to five and six years.   On Jan 1, 1981, the minimum wage increased to three dollars and thirty-five cents and it remains at the level until 1990. The current federal minimum wage has remained the same for the same amount of time. It was last increased on Sept. 1, 2010 at seven dollars and twenty-five cents. (Pollin, Robert; Wicks-Lim, Jeannette,2016).   Currently there are eighteen other states including the District of Columbia who have a higher minimum wage than what is typically required by federal law. However, there are still six states that don’t even   participate in minimum wage.   The reason why this is a global societal problem is because approximately half of the people earning the minimum wage or less are younger workers who are under the age of twenty-five.   Many of these worked still live with their parents.   Statistic show that forty-one percent stay with their patents while fifteen percent are adults, who are raising a family and twenty-three percent are adults who are barely able to take care of themselves. Although, poverty has been a huge problem for many decades (for all types of families), government officials feel that raising wages would have little effect on these group of people. Researchers have proven that various states across America have a minimum wage that is higher than what is required at the government level.   However, the federal government gives each state an opportunity to manage their minimum wage by law, but the worker will get the higher pay of the two. â€Å"Many economists do not like minimum wage for the simple fact it puts a stop to low-income workers who essentially cannot produce enough monetary worth in their work to cover the minimum wage. Often during various years such as 2008-2012, a worker in these situations has to consider the government, by looking at one’s neighborhood, education level, race, and age (24-65)† (Greory,2014).   Another factor that could be essential to help increase minimum wage is the coast of living.   Although each state has the authority to have its own minimum wage, some states are less cost efficient than others which require more than the standard minimum. â€Å"Most of the global labor force, in any case, is the developing markets.   In addition, for some of these nations, initiating a minimum wage or raising it is unequivocally on the policy agenda. Yet, little is thought about the effects of minimum wages on labor and expectations for living standards in developing markets† (Gregory, 2014).   Ã¢â‚¬Å"The cost of living takes in to account the prices of different goods and services; whereas, housing affordability considers how expensive home prices are in relation to the median family income† (Cooper,2015). However, everyone feels that things are expensive and cost a fortune. After doing research, the states that have major metro areas (such as New York and San Francisco), often gain attraction for tourist and other perks which can make it least affordable.   If the cost of living is higher than minimum wage, it increases the ability to get another job or ask for government assistance. Statics also show that 30-50% of one’s income is spent on rent and utilities, which in some cases it is 90% of their household income.   â€Å"Although some people are rent-burdened, and cannot afford basics necessities, such as fresh fruits and vegetables; so alternatively, they consume un healthy food that eventual causes health problems†. (Tsao et al 2016). Most minimum wage worker due to the lack of income cannot afford the health benefits that they need to stay healthy. Nevertheless, their ailments worsen and often time an individual may start to have feelings of depression or feeling down about their situation, this type of mindset is also unhealthy for low income Americans. Solution A probable solution to increasing minimum wages is to guarantee basic income programs like Housing Urban Development also known as HUD.   Ã¢â‚¬Å"HUD is a public housing program for all sizes and types of families, which provides housing from houses to apartments, even for the elderly. This program was developed to provide housing and community development assistance and to make sure everyone has access to â€Å"fair and equal housing† (Reich,2015).     Another opportunity for low-income families is the government assistance program that helps provide food for low income families also known as the SNAP program. This program aids with food purchases for low-income or no income families; living in the United States. SNAP benefits cost about seventy billion dollars a year and provide for about forty-four billion people.   This number represents about fourteen percent of the population, which adds up to be $125.51 per month for each person. SNAP is one of the largest nutrition programs in American; for low income families.   After reviewing the article The Effects of Minimum Wage on Food Stamps, many government officials feel that one should not have it both ways, they should not be able to collect on both benefits. Their rationale is that if minimum wages increases, that the Snap benefits decrease.   This would have an adverse effect on one’s health or possible affect the children in that household.   Government officials have spent numerus of time on discussing the issue for the welfare plan. However; since 2014, there has been an estimated increase on minimum wages and a decrease in SNAP participation, by 3.95 percent.   These results indicate that the government is going in the right direction in saving taxpayer money (Reich,2015). Although these individuals receive SNAP benefits some households still are facing the greatest hardship and most likely face a decrease in food security, rather than people who are not receiving SNAP benefits. However, SNAP enrollment seems to increase in periods of prosperity. Another solution to increasing minimum wages could be raising the child tax credit.   Families with children could really use the extra money that is provided form the benefit from child tax credit program. Many government officials are in favor of increasing the child tax credit, they say it is highly unlikely that it would be and increase in minimum wage. However, this strategy would help struggling families with children with the benefits and necessities that they need. Although most federal governments oppose of the minimum wage law they feel that there better solution for helping workers that only make minimum wage such as the earned income tax credit.   This tax credit is set up for a person who is poor and has earn a certain about due to his or her wages throughout the year.  Ã‚   The earn income tax credit has several advantages for the poor and it favors those who make minimum wage. â€Å"One argument often made by minimum wage advocates is that higher minimum wage is necessary to prevent or mitigate the reduction in market wages associated with the labor supply response to a more generous EITC† (Neumark&Washcher, 2011, p. 714).   The standard minimum wage does not cost the tax payer any money because of the higher income tax credit. This is one of the programs that was created so the government would not have to raise minimum wages, by only creating it for low income workers, who are eligible to receive it.   â€Å"Economist around the globe, feel that training programs are criticism of raising the minimum wage it is the pay rate of low-skilled people and entry level jobs†(Neumar&Wascher,2002).   The government could install training programs that could potentially improve employee outcomes and help them to be more successful in getting a high paying job. Evaluation of the Evidence The evidence used here is all justifiable and dependable and without bias. The various of sources used here are scholarly, peer-reviewed articles which make the information justifiable. There are a few sources used here that would be considered popular sources.   After reading the article, American Journal of Public Health, the authors (Tsao et al 2016) worked with the New York Department of Health and Mental Hygiene, they referenced their work and without bias. This article helped shed light on the health of low-income families. It also helped to explain how beneficial it would be if there was and increase in minimum wage and the effects it would have on and families.   The Effects of Minimum Wage on Food Stamps, is a credible scholarly source to show how the government reasons for not raising minimum wages but should create more programs to help individuals who are suffering with low-income or no income at all.   As a result, the article proved that the government is going in the right direction in saving taxpayer money.   This article is essential to research because it sheds light on the way minimum wages affect people who are less fortunate.   It also takes a closer look at those who have to use government assistance programs in order to support their families.     Statistical data provided gives rationale for raising the minimum wage and to utilize the table of data. Once again, this source is reliable, valid and unbiased. Several of the sources provide statistical data that need to be illustrated. â€Å"The statistic from this graph clearly shows the percentages of families’ income and of those who would be affected by a minimum-wage increase on a generally low to moderate level.   Nearly, 70.7 percent of affected families have a total family income of less than $60,000, and nearly a quarter (23.6 percent) have total family income of less than $20,000. Among all U.S. families, the median family income in 2010 was $60,395† (Current Population Survey, 2010) . Some of the surveys results are incorrect due to lack of education and inaccurate information being provided at the time the survey was given. The source Neumak and Wascher, shows support on finding a solution to help low-income workers.   They were able to shed light on how poverty has affected workers which have low-incomes.   Cooper explains, the percentages of youth and adults that are targeted by the fast food industries and that have no education (2015). This source explains why the government will not increase minimum wage because of the targeted age group. Ethical outcomes of Solution Some journalists have stated negative impacts, such as turnover rates and untrained workers for small businesses if wages are increased, the positive aspect of raising wages would have, overall.   After reviewing the statistical data, it shows that mostly teenagers are targeted when mentioning minimum wage, this is because they are one of the largest group that is affected by the minimum wage, which makes it effects more discernable.   After reading the article Pay Equity and the National minimum wage, the author points out that reservation wages of younger workers, which is lower than adult workers. Citizen throughout the country have had many concerns about the status of minimums wage getting abused form primary earners in poor families, more than for the wages of teenagers and other secondary earners form higher-income families. It also raises arguments on why young workers are receiving such low pay and sheds light on the national minimum wage.   The theories of wage control vary, but details pay levels and pay dispersion; thus, proving that pay differs around the world.   These differences are based on job requirements.   Younger adults are also targeted by the fast food industries because of no job experience and lack of education.   Most young adults are just trying to earn extra money rather than trying to take care of a family.   â€Å"The average age of affected workers is 36 years old. A larger share of affected workers is age 55 and older (15.3 percent) than are teens (10.7 percent). About two-thirds of affected workers are 25 years old or older† (Cooper, 2015).   There have been numerous of research obtain to show that most teenagers will not be affected by the increase because most teenagers only work part-time because they attend school. Teenagers could also learn these lessons from many other activities or discussions.   Young adults should not just rely on minimum wage; but continue their education, which would lead to an opportunity of getting a better job, when they become an adult.   They will not only have job experience but know the value of money and how it can help provide for their families.   Ã‚  There is various reason on why minimum wage should be increase because society feels like worker are less likely to quit on higher-paid jobs. Sheposh explains how minimum wage has the lowest rate of pay that was set out for mostly the fast food industry.   The government or union negotiated and calculated hourly, weekly and monthly incentives to come up with the first national minimum-wage law.   He presents the establishment and timeline of minimum wage and how it affects certain parts of the country. States like Alabama, Louisiana, Mississippi, South Carolina and Tennessee do not implement the minimum wage law.   According to this article each state and municipality can set its own wage.   â€Å"Minimum wage has always been a controversial issue and will continue to be an issue as the cost of living is always rising† (Sheposh, 2016).   However, minimum wage was not meant for workers to rely on as a permanent career, but to only use as temporary financial support.   There are many reasons that minimum wages should be increase for a worker. The standard minimum wage pay for participating states varies between $7.25 and 9.00; which in today’s society typical family of three people, would barely be able thrive.   However; from a business perspective, the employer gains profit by paying the workers less, but still has the exception for that worker to work hard and come to work every day.   In the article Santa Monica’s Minimum Wage: Assessing the Living Wage Movement’s New Frontier,it mentions that Santa Monica proposed a plan that would raise the minimum wage to $12.25 per hour (2005). After reviewing the statistical data of this source, it shows that many businesses would close due to the increase.   Studies also show that some business relocate, this is done so they will not have to pay the workers more money. It is unfair that some businesses cheat their employees out of money, so they can make more profit.   It continues to be controversial topic around the globe that the less fortunate or low-income community would be better if the government officials would come together and increase the minimum wage.   This would be beneficial, instead of developing various programs such as SNAP, earned income tax credit, and federal housing. It gives them a chance at a better life and helps them take care of their families, especially those who have unaffordable health issues.   It is immoral and unjustifiable to make someone work forty hours a week and still not be able to live.   Everyone’s situation is different but giving them the opportunity to take care of their families is morally right and ethical. If the government will increase minimum wages and keep certain government programs, then add the installation of training programs to benefit low-income and uneducated workers, they will have the opportunity to get a higher paying job.   In turn, they would no longer need the aid of federal programs; thus, this will be a great solution for the worker, as well as, the government. Minimum wages were set to only help the younger workers while in school, and other adults with no job experience. Many economists argue that by increasing minimum wage does not only help low-income worker, but all workers.   Conclusion While increasing minimum wage will always continue to be a controversial topic around the world, the wealthy have argued that the poor would be better off if the government officials would not waste their time trying to increase minimum wage.   They should instead, create more programs that will be beneficial to workers to help them get better paying jobs.   Some feel that minimum wage will reduce employment, but not by as much as there are so many people who want to work, but can not due to health issue, education, and various other issues. Increasing minimum wages has benefits for all workers, not just the one that the government targets like young workers, single adults, and worker who are 55 and older.   President Barack Obama has stated on many occasion that increasing the minimum wage is very essential to addressing the income gap in America. Raising minimum wage does not just help families financially, but it helps them feel better about themselves.   Morale would increase because they would be able to take care of their families and develop good morals and standards. However, it can help take care of health issues and not use federal programs, because they will obtain a higher paying job, which will carry health insurance and that they can afford. Making more money with just a simple increase can give a household an opportunity to be successful and take care of one’s family.   Poverty in America is at an all-time high and the lower-class has been affected by the increase in the cost of living. We have to find a way for government officials to make the decision to increase minimum wages. References Brosnan,  P. (n.d.). The Minimum Wage in a Global Context.  The Handbook of Globalization, Second Edition. doi:10.4337/9780857931443.00020 Cooper, D. (2015). Raising the Minimum Wage to $12 by 2020 Would Lift Wages for 35 Million American Workers | Economic Policy Institute. Retrieved from http://www.epi.org/publication/raising-the-minimum-wage-to-12-by-2020-would-lift-wages-for-35-million-american-workers/ Doyle, A. 14 Dec 2017. Pros and Cons of Raising Minimum Wage. Web. https://www.thebalance.com/pros-and-cons-of-raising-the-minimum-wage-2062521 Edwards, P., & Gilman, M. (1999). Pay equity and the national minimum wage: What can theories tell us? Human Resource Management Journal, 9(1), 20-38. Retrieved from http://search.proquest.com/docview/199448047?accountid=32521 Gregory,  T. (2014). When the Minimum Wage Bites Back: Quantile Treatment Effects of a Sectoral Minimum Wage in Germany.  SSRN Electronic Journal.  doi:10.2139/ssrn.2567167 Meer, J., & West, J. (2016). Effects of the Minimum Wage on Employment Dynamics. Journal Of Human Resources, 51(2), 500-522. Neumark, D., & Wascher, W. (2002). Do Minimum Wages Fight Poverty. Economic Inquiry, 40(3), 315. Papworth, J. 26 Oct. 2012. How Good is Great Britian’s Minimum Wage? Web.   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   https://www.theguardian.com/money/2012/oct/26/how-good-britain-minimum-wage Pollin, Robert; Wicks-Lim, Jeannette.   A $15 U.S. minimum wage: How fast-food industry could adjust without shedding jobs.   Journal of Economic Issues (M.E. Sharpe Inc.).  Sep2016, Vol. 50 Issue 3, p716-744. 29p. 10 Charts. DOI: 10.1080/00213624.2016.1210382 Reich, Michael.  Industrial Relations. â€Å"The Effects of Minimum Wage on Food Stamps†.  Oct2015, Vol. 54 Issue 4, p668-694. 27p. 8 Charts, 1 Map DOI: 10.1111/irel.12105. Sanders, R. H., & Williams, E. D. (2005, February). Assessing the Living Wage Movement’s  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   New Frontier. Santa Monica’s Minimum Wage:, 19(1). doi:10.1177/0891242404268705 Sheposh, R. January,2016 2p. â€Å"Minimum Wages around the World† Salem Press. Web. 26 Jan. 2016. http://www.wageindicator.org/main/salary/minimum-wageontier. Santa Monica’s Minimum Wage:, 19(1). doi:10.1177/0891242404268705 Tsao et al. (June 2016). Estimating potential reductions in premature mortality in New York City from raising the minimum wage to $15. American Journal of Public Health, 106(6): 1036-1041